تفاصيل الوثيقة

نوع الوثيقة : مقال في مجلة دورية 
عنوان الوثيقة :
pecific nutrient combination effects on tax, NF- κB and MMP-9 in human T-cell lymphotropic virus -1 positive malignant T-lymphocytes
pecific nutrient combination effects on tax, NF- κB and MMP-9 in human T-cell lymphotropic virus -1 positive malignant T-lymphocytes
 
لغة الوثيقة : الانجليزية 
المستخلص : BACKGROUND: Adult T-cell Leukemia (ATL) is a disease with no known cure. The disease manifests itself as an aggressive proliferation of CD4+ cells with the human T-cell Lymphotropic virus type 1 (HTLV-1). The leukemogenesis of the virus is mainly attributed to the viral oncoprotein. Tax activates the Nuclear Factor kappa B (NF-κB) which stimulates the activity and expression of the matrix metalloproteinase-9 (MMP-9). The objective of this study was to investigate the efficacy of a specific nutrient synergy (SNS) on proliferation, Tax expression, NF-κB levels as well as on MMP-9 activity and expression both at the transcriptional and translational levels in two HTLV-1 positive cell lines, HuT-102 and C91-PL at 48h and 96h of incubation. Cytotoxicity of Epigallocatechin-3-gallate (EGCG) was assayed using CytoTox 96 Non-radioactive and proliferation was measured using Cell Titer96TM Nonradioactive Cell Proliferation kit (MTT- based assay). Enzyme linked immunosorbant assay (ELISA) and electrophoretic mobility shift assay (EMSA) were used to assess the effect of SNS on NF-κB mobility. Zymography was used to determine the effects of SNS on the activity and secretion of MMP-9. The expression of MMP-9 was done using RT-PCR at the translational level and Immunoblotting at the transcriptional level. RESULTS: A significant inhibition of proliferation was seen in both cell lines starting at a concentration of 200μg/ml and in a dose dependent manner. SNS induced a dose dependent decrease in Tax expression, which was paralleled by a down-regulation of the nuclearization of NF-κB. This culminated in the inhibition of the activity of MMP-9 and their expression both at the transcriptional and translational levels. CONCLUSIONS: The results of this study indicate that a specific nutrient synergy targeted multiple levels pertinent to the progression of ATL. Its activity was mediated through the NF-κB pathway, and hence has the potential to be integrated in the treatment of this disease as a natural potent anticancer agent. 
ردمد : 1471-2407 
اسم الدورية : BMC Cancer 
المجلد : 15 
العدد : 1 
سنة النشر : 1436 هـ
2015 م 
نوع المقالة : مقالة علمية 
تاريخ الاضافة على الموقع : Wednesday, April 20, 2016 

الباحثون

اسم الباحث (عربي)اسم الباحث (انجليزي)نوع الباحثالمرتبة العلميةالبريد الالكتروني
Steve HarakehHarakeh, Steve باحث رئيسي  
Rania AzarAzar, Rania باحث مشارك  
Esam AzharAzhar, Esam باحث مشارك  
Ghazi A. DamanhouriDamanhouri, Ghazi A.باحث مشارك  
Mourad AssidiAssidi, Mourad باحث مشارك  
Muhammad Abu-ElmagdAbu-Elmagd, Muhammad باحث مشارك  
Mohammed H. AlqahtaniAlqahtani, Mohammed H.باحث مشارك  
Taha KumosaniKumosani, Taha باحث مشارك  
Aleksandra NiedzwieckiNiedzwiecki, Aleksandra باحث مشارك  
Mathias RathRath, Mathias باحث مشارك  
Ahmed Al-HejinAl-Hejin, Ahmed باحث مشارك  
Elie BarbourBarbour, Elie باحث مشارك  
Mona Diab-AssafDiab-Assaf, Mona باحث مشارك  

الملفات

اسم الملفالنوعالوصف
 38630.pdf pdf 

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