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نوع الوثيقة : مقال في مجلة دورية 
عنوان الوثيقة :
Fast solubilization of recalcitrant cellulosic biomass by the basidiomycete fungus Laetisaria arvalis involves successive secretion of oxidative and hydrolytic enzymes
Fast solubilization of recalcitrant cellulosic biomass by the basidiomycete fungus Laetisaria arvalis involves successive secretion of oxidative and hydrolytic enzymes
 
لغة الوثيقة : الانجليزية 
المستخلص : Background: Enzymatic breakdown of lignocellulosic biomass is a known bottleneck for the production of high-value molecules and biofuels from renewable sources. Filamentous fungi are the predominant natural source of enzymes acting on lignocellulose. We describe the extraordinary cellulose-deconstructing capacity of the basidiomycete Laetisaria arvalis, a soil-inhabiting fungus. Results: The L. arvalis strain displayed the capacity to grow on wheat straw as the sole carbon source and to fully digest cellulose filter paper. The cellulolytic activity exhibited in the secretomes of L. arvalis was up to 7.5 times higher than that of a reference Trichoderma reesei industrial strain, resulting in a significant improvement of the glucose release from steam-exploded wheat straw. Global transcriptome and secretome analyses revealed that L. arvalis produces a unique repertoire of carbohydrate-active enzymes in the fungal taxa, including a complete set of enzymes acting on cellulose. Temporal analyses of secretomes indicated that the unusual degradation efficiency of L. arvalis relies on its early response to the carbon source, and on the finely tuned sequential secretion of several lytic polysaccharide monooxygenases and hydrolytic enzymes targeting cellulose. Conclusions: The present study illustrates the adaptation of a litter-rot fungus to the rapid breakdown of recalcitrant plant biomass. The cellulolytic capabilities of this basidiomycete fungus result from the rapid, selective and successive secretion of oxidative and hydrolytic enzymes. These enzymes expressed at critical times during biomass degradation may inspire the design of improved enzyme cocktails for the conversion of plant cell wall resources into fermentable sugars. 
ردمد : 1754-6834 
اسم الدورية : BIOTECHNOLOGY FOR BIOFUELS 
المجلد : 7 
العدد : 143 
سنة النشر : 1435 هـ
2014 م 
نوع المقالة : مقالة علمية 
تاريخ الاضافة على الموقع : Thursday, August 10, 2017 

الباحثون

اسم الباحث (عربي)اسم الباحث (انجليزي)نوع الباحثالمرتبة العلميةالبريد الالكتروني
David NavarroNavarro, David باحث  
Marie-Noelle RossoRosso, Marie-Noelle باحث  
Mireille HaonHaon, Mireille باحث  
Caroline OliveOlive, Caroline باحث  
Estelle BonninBonnin, Estelle باحث  
Laurence Lesage-MeessenLesage-Meessen, Laurence باحث  
Didier ChevretChevret, Didier باحث  
Pedro CoutinhoCoutinho, Pedro باحث  
Bernard HenrissatHenrissat, Bernard باحث  
Jean-Guy BerrinBerrin, Jean-Guy باحث  

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 42556.pdf pdf 

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