تفاصيل الوثيقة

نوع الوثيقة : مقال في مجلة دورية 
عنوان الوثيقة :
Dissection of Hexosyl- and Sialyltransferase Domains in the Bifunctional Capsule Polymerases from Neisseria meningitidis Wand Y Defines a New Sialyltransferase Family
Dissection of Hexosyl- and Sialyltransferase Domains in the Bifunctional Capsule Polymerases from Neisseria meningitidis Wand Y Defines a New Sialyltransferase Family
 
لغة الوثيقة : الانجليزية 
المستخلص : Background: Capsule polymerases of Neisseria meningitidis serogroups W and Y comprise hexosyl- and sialyltransferase activity. Results: Hexosyltransferase activity is encoded by the predicted N-terminal GT-B fold. Sialyltransferase activity requires 168 additional amino acids upstream of the predicted C-terminal GT-B fold. Conclusion: The sialyltransferase domains of NmW/Y define a new glycosyltransferase (CAZy) family. Significance: The new CAZy family comprises sequences from distantly related species. Crucial virulence determinants of disease causing Neisseria meningitidis species are their extracellular polysaccharide capsules. In the serogroups W and Y, these are heteropolymers of the repeating units (6)--d-Gal-(14)--Neu5Ac-(2)(n) in NmW and (6)--d-Glc-(14)--Neu5Ac-(2)(n) in NmY. The capsule polymerases, SiaD(W) and SiaD(Y), which synthesize these highly unusual polymers, are composed of two predicted GT-B fold domains separated by a large stretch of amino acids (aa 399-762). We recently showed that residues critical to the hexosyl- and sialyltransferase activity are found in the predicted N-terminal (aa 1-398) and C-terminal (aa 763-1037) GT-B fold domains, respectively. Here we use a mutational approach and synthetic fluorescent substrates to define the boundaries of the hexosyl- and sialyltransferase domains. Our results reveal that the active sialyltransferase domain extends well beyond the predicted C-terminal GT-B domain and defines a new glycosyltransferase family, GT97, in CAZy (Carbohydrate-Active enZYmes Database). 
ردمد : 0021-9258 
اسم الدورية : JOURNAL OF BIOLOGICAL CHEMISTRY 
المجلد : 289 
العدد : 49 
سنة النشر : 1435 هـ
2014 م 
نوع المقالة : مقالة علمية 
تاريخ الاضافة على الموقع : Thursday, August 10, 2017 

الباحثون

اسم الباحث (عربي)اسم الباحث (انجليزي)نوع الباحثالمرتبة العلميةالبريد الالكتروني
Angela RomanowRomanow, Angela باحث  
Timothy KeysKeys, Timothy باحث  
Katharina StummeyerStummeyer, Katharina باحث  
Friedrich FreibergerFreiberger, Friedrich باحث  
Bernard HenrissatHenrissat, Bernard باحث  

الملفات

اسم الملفالنوعالوصف
 42560.pdf pdf 

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