تفاصيل الوثيقة

نوع الوثيقة : مقال في مجلة دورية 
عنوان الوثيقة :
Complete genome sequence of DSM 30083(T), the type strain (U5/41(T)) of Escherichia coli, and a proposal for delineating subspecies in microbial taxonomy
Complete genome sequence of DSM 30083(T), the type strain (U5/41(T)) of Escherichia coli, and a proposal for delineating subspecies in microbial taxonomy
 
لغة الوثيقة : الانجليزية 
المستخلص : Although Escherichia coli is the most widely studied bacterial model organism and often considered to be the model bacterium per se, its type strain was until now forgotten from microbial genomics. As a part of the Genomic Encyclopedia of Bacteria and Archaea project, we here describe the features of E. coli DSM 30083(T) together with its genome sequence and annotation as well as novel aspects of its phenotype. The 5,038,133 bp containing genome sequence includes 4,762 protein-coding genes and 175 RNA genes as well as a single plasmid. Affiliation of a set of 250 genome-sequenced E. coli strains, Shigella and outgroup strains to the type strain of E. coli was investigated using digital DNA: DNA-hybridization (dDDH) similarities and differences in genomic G+C content. As in the majority of previous studies, results show Shigella spp. embedded within E. coli and in most cases forming a single subgroup of it. Phylogenomic trees also recover the proposed E. coli phylotypes as monophyla with minor exceptions and place DSM 30083T in phylotype B2 with E. coli S88 as its closest neighbor. The widely used lab strain K-12 is not only genomically but also physiologically strongly different from the type strain. The phylotypes do not express a uniform level of character divergence as measured using dDDH, however, thus an alternative arrangement is proposed and discussed in the context of bacterial subspecies. Analyses of the genome sequences of a large number of E. coli strains and of strains from > 100 other bacterial genera indicate a value of 79-80% dDDH as the most promising threshold for delineating subspecies, which in turn suggests the presence of five subspecies within E. coli. 
ردمد : 1944-3277 
اسم الدورية : STANDARDS IN GENOMIC SCIENCES 
المجلد : 9 
العدد : 1 
سنة النشر : 1435 هـ
2014 م 
نوع المقالة : مقالة علمية 
تاريخ الاضافة على الموقع : Monday, August 14, 2017 

الباحثون

اسم الباحث (عربي)اسم الباحث (انجليزي)نوع الباحثالمرتبة العلميةالبريد الالكتروني
Jan Meier-KolthoffMeier-Kolthoff, Jan باحث  
Richard HahnkeHahnke, Richard باحث  
Joern PetersenPetersen, Joern باحث  
Carmen ScheunerScheuner, Carmen باحث  
Victoria MichaelMichael, Victoria باحث  
Anne FiebigFiebig, Anne باحث  
Christine RohdeRohde, Christine باحث  
Manfred RohdeRohde, Manfred باحث  
Berthold FartmannFartmann, Berthold باحث  
Lynne GoodwinGoodwin, Lynne باحث  
Olga ChertkovChertkov, Olga باحث  
T ReddyReddy, T باحث  
Amrita PatiPati, Amrita باحث  
Natalia IvanovaIvanova, Natalia باحث  
Victor MarkowitzMarkowitz, Victor باحث  
Nikos KyrpidesKyrpides, Nikos باحث  
Tanja WoykeWoyke, Tanja باحث  
Markus GoekerGoeker, Markus باحث  
Hans-Peter KlenkKlenk, Hans-Peter باحث  

الملفات

اسم الملفالنوعالوصف
 42619.pdf pdf 

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