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Document Details
Document Type
:
Thesis
Document Title
:
Molecular Detection and typing of group A rotavirus among hospitalized children in Makkah
الكشف الجزيئي والتصنيفي للمجموعة أ لفيروس الروتا لدى الأطفال المنومين في مستشفيات مكة
Subject
:
Biochemistry department
Document Language
:
Arabic
Abstract
:
Group A Rotavirus is the leading cause of severe childhood diarrhea worldwide, resulting in ~ 600,000 deaths annually in children <5 years of age. Rotavirus infection is highly contagious and not easily controlled by improvements in hygiene and sanitation, as evidenced by similar incidence rates in developed and developing nations. The only control measure likely to have a significant impact on the incidence of severe disease is vaccination, but circulating rotaviruses are antigenically and genotypically diverse. The genotypic diversity of circulating rotavirus strains needs to be assessed before, during, and after vaccination of populations. Detection and genotyping of group A rotavirus strains from stool samples in young children with diarrhea in Makkah were examined using ElISA and genotyping by reverse transcription-nested multiplex PCR. Total Of 173 stool specimens, 75 samples was rotavirus positive. G1 type was the most common G-type (46.6%), followed by G2 (14.67%) ,G9 (6.6%), G3 (62.6%) G4 (1.3%) and G8 (1.3%). P[8] was the most common P-type (53.3%), followed by P[6] (14.6%) and P[4] (10.6%) .The prevalence of G-P combination type was genotype G1P[8] (46.6%), followed by G9P[8] (10.6%), G12P[8] (8%), G2P[6] (8%) G2P[4] (6%) G1P[6] (8%) G1P[4] (8%) G8P[8] (8%) G3P[8] (8%) G2P[8] (8%) G4P[8] (8%).
Supervisor
:
Prof.Taha A. kumosani
Thesis Type
:
Master Thesis
Publishing Year
:
1433 AH
2011 AD
Co-Supervisor
:
Dr. Esam I. Azhar
Added Date
:
Tuesday, December 13, 2011
Researchers
Researcher Name (Arabic)
Researcher Name (English)
Researcher Type
Dr Grade
Email
نوره عبدالرؤوف القايدي
AL-kaiedi, Norah Abdulraouf
Researcher
Master
Files
File Name
Type
Description
31666.pdf
pdf
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