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Deanship of Graduate Studies
Document Details
Document Type
:
Thesis
Document Title
:
CHARACTERIZATION OF NOVEL DIBENZOFURAN DEGRADING BACTERIA FROM MAKKAH REGION.
توصيف نوع جديد من البكتريا لها القدرة على تحليل مادة الدايبنزوفيوران من منطقة مكة المكرمة
Subject
:
faculty of science
Document Language
:
Arabic
Abstract
:
Dibenzofuran mostly found in the chlorinated form are omnipresent in the environment endangering normal flora, fauna and posing a risk to human health. Bioremediation is one of the processes that could alleviate the polluted soil of contamination. The study aimed to identify native bacterial strains present in soils around Makkah region that degrade dibenzofuran. Samples collected from the different region was used to isolate bacterial species capable of degrading this environmentally hazardous chemical. Bacterial strains designated as DF9, DF2 and DF6 were selected for qualitative analysis such as DCPIP and spectrophotometer. DF9 was selected for the quantitative analysis using HPLC. Furthermore, the selected bacterial strains were identified using biochemical and Molecular technique. Results show that all of the three selected samples change its color from blue to white in the presence of DCPIP dye compare to control. Using UV spectrophotometer the selected samples also show high optical density at 600 nm. Result of HPLC (High-performance liquid chromatography) analysis of DF9 confirms its degradative ability as it degrades almost 100 % of dibenzofuran compared to the control one. It is concluded from our studies that these strains can be used to clean the environment from this hazardous chemical.
Supervisor
:
Dr. Yasir Anwar Ulhaq
Thesis Type
:
Master Thesis
Publishing Year
:
1441 AH
2019 AD
Co-Supervisor
:
Dr. Hani Mohammed Ali
Added Date
:
Monday, December 9, 2019
Researchers
Researcher Name (Arabic)
Researcher Name (English)
Researcher Type
Dr Grade
Email
محمد هاشم القثامى
Alqethami, Mohammed
Researcher
Master
Files
File Name
Type
Description
45658.pdf
pdf
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